[all at arabidopsis.uk] NASC order ID transaction Authorised

Yue Zhou yue_zhou at pku.edu.cn
Thu Jan 14 02:18:50 GMT 2021 

To whom it may concern,

I have received the seeds (except CS93526). As I payed by my credit card, could you please give me a receipt? My order ID is ID58420. Thanks. 
 
Best,
Yue
---------------------
Yue Zhou, PhD
Group of Epigenetics and Plant Development
State Key Laboratory for Protein and Plant Gene Research
Peking-Tsinghua Center for Life Sciences
School of Advanced Agricultural Sciences
Peking University
http://www.cls.edu.cn/english/PrincipalInvestigator/pi/index4282.shtml

> 2020年11月17日 18:33，Emma Wigmore <Emma.Wigmore at nottingham.ac.uk> 写道：
> 
> Dear Yue,
>  
> Thank you for the confirmation. I have placed an order on your behalf for the stocks you listed. You should receive a confirmation email, but I have pasted the details below. Please let me know if anything needs changing. I have made a note on the system that you have paid by credit card so you will not be charged twice.
>  
> Apologies again for the inconvenience caused,
>  
> Emma
>  
> Dr Emma Wigmore
> Nottingham Arabidopsis Stock Centre
> School of Biosciences
> University of Nottingham
> Sutton Bonington Campus
> Loughborough
> LE12 5RD
> United Kingdom
> 
> Tel: +44 (0)115 95 13091
>  
> Ordering
> 
> Your invoice request has been successful
> Your NASC order ID is 58420
> Please quote your order ID in any future correspondence
> To check the progress of your order please use our order tracking page <http://arabidopsis.info/cgi-bin/order/order_tracking.pl>.
>  
> Order Summary
>  
> Basket Contents
> Stock Code
> Name
> Collection
> Description
> Cost
> N93526
> Tilling line
> Tilling
> Individual line descended from individual M2 plant from ethyl-methane sulfonate (EMS) mutagenesis. Individual lines should be ordered from this population as followup to positive results in the TILLING (Targeting Induced Local Lesions IN Genomes) procedure conducted by the Henikoff laboratory. The reverse genetics, TILLING procedure employs methods which detect heteroduplex DNA for targeted DNA sequences so that EMS-induced point mutations in specific genes of interest can be identified. Details of EMS treatment, screening strategy are provided in the references associated with this resource. All lines are M3 generation, distribution seeds are original donation.
> £10.00
> N420954
> GK-219C02
> GABI-Kat
> THIS IS A GABI KAT SET COMPRISING OF A NUMBER OF INDIVIDUAL LINES. ALL INDIVIDUAL LINES ARE INCLUDED IN EACH SET AND YOU WILL NEED ALL INDIVIDUAL LINES TO DETERMINE WHICH LINE IN THE SET IS HOMOZYGOUS. GABI-KAT HAVE CONFIRMED THAT WITHIN EACH SET THERE IS A HOMOZYGOUS LINE BUT THEY DO NOT KNOW WHICH INDIVIDUAL LINE IT IS. THEREFORE YOU NEED TO DETERMINE THIS YOURSELF. ALL SET CODES BEGIN WITH N4. ALL INDIVIDUAL LINES CODES BEGIN WITH EITHER N2, N3 or N7. PARENTAL LINE SELECTED FROM THE ORIGINAL TRANSFORMANT.
> £15.00
> N543300
> SALK_043300
> SALK segregating lines
> Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. 
> Associated Polymorphisms: 
> SALKseq_043300.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G51745.1 (at chr 1 pos 19191975 (W/19191910-19191975) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 13, R 15, C 13 N 12. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO368232.
> £10.00
> N670694
> SALK_049122C
> Homozygous lines
> SALK homozygous line from original SALK line N549122
> £10.00
> N836957
> SAIL_828_A07
> SAIL
> T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. 
> £10.00
> N849700
> WiscDsLox247F07
> Wisconsin DsLox
> Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. 
> Associated Polymorphisms: 
> Wiscseq_DsLox247F07.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G19740.1-P355 and AT4G19750.1 (chr 4 pos 10745408 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796634. 
> Wiscseq_DsLox247F07.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G21700.1 (at chr 1 pos 7622136 (C/7622136-7622235) on the TAIR10). The sequences' 1-100 bps mapped on the genome. Pools P 0, R 6, C 8 N 0. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452992.
> £10.00
> Subtotal
> £65.00
> Batch fee <http://www.arabidopsis.info/InfoPages?template=fees;web_section=germplasm>	
> £10.00
> VAT amount
> £0.00
> Total
> £75.00
> 致中国顾客
> 
> 为了确保您的订单快速，安全地交付，请在下面的框中保存您的中文地址。您也可以通过电子邮件将您的姓名和地址以中文发送至：all at arabidopsis.uk <mailto:all at arabidopsis.uk>.
> MESSAGE TO CUSTOMERS IN CHINA
> 
> To ensure a fast and safe delivery of your order please save your address in Chinese in the box below.You can also send your name and address in Chinese by email to all at arabidopsis.uk <mailto:all at arabidopsis.uk>
> Name:
> Dr Yue Zhou
> Institution:
> National Laboratory of Protein Engineering and Plant Genetic Engineering,College of Life Sciences,Peking University
> Address:
> 5 Yiheyuan Road ,Haidian District,Beijing,Peking,Beijing,100871,CHINA
> Email address:
> yue_zhou at pku.edu.cn <mailto:yue_zhou at pku.edu.cn>
> Telephone:
> 0086-10-62754535
> Fax:
> Head of Group:
> Yue Zhou
> Purchase order number:
> Order paid for on credit card EJW
>  
>  
>  
> From: Yue Zhou <yue_zhou at pku.edu.cn>
> Date: Tuesday, 17 November 2020 at 09:41
> To: Emma Wigmore <sbzejh at exmail.nottingham.ac.uk>
> Subject: Re: [all at arabidopsis.uk] NASC order ID transaction Authorised
> 
> Dear Emma,
>  
> Thank you for your reply. Here is the order details:
> GK-219C02
> CS93526
> SALK_043300
> SALK_049122C
> WiscDsLox247F07
> SAIL_828_A07
>  
> My name is Yue Zhou, I work as a PI in Peking University.
> Best,
> Yue
> ---------------------
> Yue Zhou, PhD
> Group of Epigenetics and Plant Development
> State Key Laboratory for Protein and Plant Gene Research
> Peking-Tsinghua Center for Life Sciences
> School of Advanced Agricultural Sciences
> Peking University
> http://www.cls.edu.cn/english/PrincipalInvestigator/pi/index4282.shtml <http://www.cls.edu.cn/english/PrincipalInvestigator/pi/index4282.shtml>
> 
> 
> 2020年11月17日 16:54，Emma Wigmore <Emma.Wigmore at nottingham.ac.uk <mailto:Emma.Wigmore at nottingham.ac.uk>> 写道：
> 
> Dear Yue,
>  
> Thank you for your email. It appears there was a problem with your order and it hasn’t reached our database, apologies.
>  
> Could you send me the list of stocks you require and the shipping name and address?
>  
> Best wishes
>  
> Emma
>  
> Dr Emma Wigmore
> Nottingham Arabidopsis Stock Centre
> School of Biosciences
> University of Nottingham
> Sutton Bonington Campus
> Loughborough
> LE12 5RD
> United Kingdom
> 
> Tel: +44 (0)115 95 13091
>  
> From: Yue Zhou <yue_zhou at pku.edu.cn <mailto:yue_zhou at pku.edu.cn>>
> Date: Tuesday, 17 November 2020 at 00:30
> To: all at arabidopsis.uk <mailto:all at arabidopsis.uk> <all at arabidopsis.uk <mailto:all at arabidopsis.uk>>
> Subject: [all at arabidopsis.uk <mailto:all at arabidopsis.uk>] Re: NASC order ID transaction Authorised
> 
> To whom it may concern,
> 
> I ordered seeds from NASC and paid by my credit. Then I received the email with the context as below. May I know my order work or not.?
> 
> Best,
> Yue
> ---------------------
> Yue Zhou, PhD
> Group of Epigenetics and Plant Development
> State Key Laboratory for Protein and Plant Gene Research
> Peking-Tsinghua Center for Life Sciences
> School of Advanced Agricultural Sciences
> Peking University
> http://www.cls.edu.cn/english/PrincipalInvestigator/pi/index4282.shtml <http://www.cls.edu.cn/english/PrincipalInvestigator/pi/index4282.shtml>
> 
> > 2020年11月17日 08:26，Arabidopsis <do-not-reply at worldpay.com> 写道：
> > 
> > Your NASC order - order ID T261e92263e46394912c4ebaf73c1d8d0 has been authorised.
> > 
> > Thank you for your order
> > Your payment is securely processed by WorldPay.
> 
> This message and any attachment are intended solely for the addressee
> and may contain confidential information. If you have received this
> message in error, please contact the sender and delete the email and
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> 
> Any views or opinions expressed by the author of this email do not
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> communications with the University of Nottingham may be monitored 
> where permitted by law.
> 
> 
> 
> 
> 
>  
> This message and any attachment are intended solely for the addressee
> and may contain confidential information. If you have received this
> message in error, please contact the sender and delete the email and
> attachment. 
> 
> Any views or opinions expressed by the author of this email do not
> necessarily reflect the views of the University of Nottingham. Email
> communications with the University of Nottingham may be monitored 
> where permitted by law.
> 
> 
> 
> 

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