[Syrphidae] Preparing syrphid eggs for SEM

Chris Bergh cbergh@vt.edu
Fri, 23 May 2003 08:38:47 -0400


Good morning,

We wish to photograph some syrphid eggs using SEM and would like to know
whether any of you have done this before? If so, is there an accepted
protocol for preparing them prior to sputter coating? Does the standard
gluteraldehyde plus alcohol series work or are there other techniques that
work better? Any feedback would be gratefully received. Thanks. - Chris
Bergh and Brent Short
J. Christopher Bergh
Virginia Tech
Alson H. Smith, Jr. AREC
595 Laurel Grove Road
Winchester, VA 22602
Tel: (540) 869-2560 ext. 32
Fax: (540) 869-0862